Ssl2 protein in PIC complex

TSS mutations I screened are:

About

I'm a Research Scientist with a background in both Molecular Biology and Bioinformatics. I obtained my PhD degree in Dr. Craig Kaplan's group at University of Pittsburgh (Pitt). During my graduate school study, I dissected Ssl2/TFIIH protein's function in RNA Polymerase II (Pol II) transcription start site (TSS) selection using yeast genetics and NGS methods. After Pitt, I joined Brigham and Women's Hospital as a Research Scientist in Bioinformatics and worked on next-generation sequencing (NGS) data analysis, mainly in single-cell RNA-sequencing data analysis. My expertise involves both dry lab and wet lab skills (moist). Currently, I'm highly interested in using various technologies to solve biological questions that people are most caring about, cancer.

Skills

The highlights of my skills are in molecular biology and NGS data analysis. I've also trained by the best Geneticist, my PhD advisor Dr. Craig Kaplan, to learn the power and beauty of the Genetics in solving biological questions. In addition, I love teaching and enjoy the process of interpreting and sharing research with others.

Molecular Biology

  • DNA/RNA manipulation
  • Molecular cloning
  • Genome editing/CRISPR
  • In vitro protein expression & purification
  • NGS library preparation

Bioinformatics

  • NGS data analysis
  • scRNA-seq data analysis
  • scATAC-seq data analysis
  • Super computing
  • AWS

Teaching

  • Pitt Bio Gene-team
  • Biochemistry recitation
  • Bioinformatics

Resume

Education

Doctorate of Science & Biological Sciences

2013 - 2019

University of Pittsburgh, Pittsburgh, PA

Advisor: Craig Kaplan

Thesis: Mechanistic insights into the Function of Ssl2/TFIIH in RNA Polymerase II Transcription Start Site Scanning.

Master of Science & Cell Biology

2009 - 2012

Amoy University, Xiamen, China

Advisor: Liang Chen

Thesis: Functional Identification and Expression Analysis in Stress Responses of a NB-ARC Domain Containing Protein OsPDRH9N.

Bachelor of Engineering & Biological Technology

2005 - 2009

Lanzhou University of Technology, Lanzhou, China

Advisor: Xueyan Li

Thesis: Culture condition tests of beer originated Lactobacillus and its exopolysaccharide (EPS) analysis by Thin-layer chromatography (TLC).

Professional Experience

Research Scientist in Medical Oncology

2022 - current

Dana-Farber Cancer Institute, Boston, MA

  • I study cancer.

Research Scientist in Bioinformatics

2021 - 2022

Brigham and Women's Hospital, Boston, MA

  • scRNA-seq data analysis for cell type and gene expression investigation.

PhD and Postdoctoral Associate

2013 - 2019

University of Pittsburgh, Pittsburgh, PA

  • RNA polymerase II transcription initiation and transcription start site selection.

Research Assistant

2012 - 2013

National Institute of Biological Sciences, Beijing, China

Publications

Archived projects

Conferences

Teaching & Mentoring

Science Gallery

  • All
  • Yeast
  • Molecular biology
  • Genomics

Growing random thoughts

  • 2022, year of changing.

    Learning new skills is always good, however, chasing on new technologies is not wise. Mastering how to use diverse technologies into a project for solving a practical question is the goal of my next career stage.

  • 2019, year of graduation.

    Learning is an endless process, keep learning but also thinking about finding a "place" to land what I have learned.